eBook
JUN 03, 2024 11:15 AM PDT

Centrifuge-Free Clarification and Harvest of AAV

SPONSORED BY: Sartorius
You don't want to miss this!
To permanently access this eBook for free, fill out the short form below.
Unlock eBook
ALREADY ACCESSED THIS eBook? SIGN IN

This application note highlights a study that evaluates the use of Sartoclear Dynamics® Lab Filter Aid, containing diatomaceous earth (DE), for clarifying crude lysates of adeno-associated virus (AAV) produced by HEK293 suspension cells. 

The data shows that filtration using Sartoclear Dynamics® Lab Filter Aid is a viable clarification method for AAV2, AAV5 and AAV8. For standard AAV production processes, AAV particle recovery in the filtrate was >95%. Even difficult-to-handle serotypes such as AAV2 and lysates containing lower AAV particle titers could be clarified by Sartoclear Dynamics® Lab filtration with viral recoveries of >80%. Moreover, the use of Sartoclear Dynamics® Lab Filter Aid reduced manual handling time 3-fold and increased the filter capacity 3.5-fold compared to the standard laboratory method of filtration combined with a prior centrifugation step.

Learn more about the methodology in this application note.


You May Also Like
Loading Comments...