mouse monoclonal IgG2a; HPV16 E7 Antibody (TVG710Y) is an IgG2a κ mouse monoclonal HPV16 E7 antibody that detects E7 of HPV-16 origin by WB, IP and IF. HPV16 E7 Antibody (TVG710Y) is available as the non-conjugated anti-HPV16 E7 antibody form, as well as multiple conjugated forms of anti-HPV16 E7 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The HPV E7 proteins are small zinc-binding phosphoproteins that are localized in the nucleus. They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses. The CR2 homology region contains the LXCXE motif (residues 22-26) involved in binding to the tumor suppressor protein pRb. This sequence is also present in SV40 and polyoma large T antigens. The high risk HPV E7 proteins (e.g. HPV16 E7 and HPV18 E7) have an approximately ten-fold higher affinity for pRb protein than the low risk HPV E7 proteins (e.g. HPV6 E7). Association of the E7 protein with pRb promotes cell proliferation by the same mechanism as the E1A proteins of adenoviruses and SV40 large T antigen. Research has shown that E7 promotes degradation of Rb family proteins rather than simply inhibiting their function by complex formation. The CR2 region also contains the casein kinase II phosphorylation site (residues 31 and 32). HPV16 and 18 are strongly associated with cervical, vaginal and vulvar malignancies.