mouse monoclonal IgG2a; Raf-B Antibody (F-7) is an IgG2a κ mouse monoclonal Raf-B antibody (also designated BRAF antibody or RAFB antibody) that detects the Raf-B protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Raf-B Antibody (F-7) is available as both the non-conjugated anti-Raf-B antibody form, as well as multiple conjugated forms of anti-Raf-B antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.