mouse monoclonal IgG1; Wee 1 Antibody (B-11) is an IgG1 κ mouse monoclonal Wee 1 antibody (also designated WEE1 antibody) that detects the Wee 1 protein of human origin by WB, IP, IF, IHC(P) and ELISA. Wee 1 Antibody (B-11) is available as both the non-conjugated anti-Wee 1 antibody form, as well as multiple conjugated forms of anti-Wee 1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Phosphorylation of Cdc2 on threonine 14 and tyrosine 15 is required to maintain Cdc2 in an inactive state throughout the S and G2 phases of the cell cycle. The human Wee 1 protein, Wee 1 Hu, encodes a tyrosine-specific protein kinase that phosphorylates Cdc2 on tyrosine 15. Myt 1, a member of the Wee 1 family of protein kinases, has been shown to phosphorylate Cdc2 on both threonine 14 and tyrosine 15 in a cyclin-dependent manner. Activity of both Wee 1 Hu and Myt 1 is regulated during the cell cycle, suggesting that both proteins play a role in mitotic control. Dephosphorylation of Cdc2 on threonine 14 and tyrosine 15 in late G2 by Cdc25 then activates the Cdc2/cyclin B complex to allow entry into mitosis.