mouse monoclonal IgG2b; PLC β1 Antibody (D-8) is an IgG2b κ mouse monoclonal PLC beta 1 antibody (also designated Phospholipase C Beta 1 antibody, PLC-I antibody, 1-Phosphatidylinositol 45-Bisphosphate Phosphodiesterase Beta-1 antibody, Phospholipase C Beta 1 antibody, PLC-Beta-1 antibody, 1-Phosphatidyl-D-Myo-Inositol-45-Bisphosphate antibody, Monophosphatidylinositol Phosphodiesterase antibody, Phosphoinositide Phospholipase C-Beta-1 antibody, Triphosphoinositide Phosphodiesterase antibody, Inositoltrisphosphohydrolase antibody, Phospholipase C-Beta-1 antibody, or Phosphoinositidase C antibody) that detects the PLC beta 1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. PLC β1 Antibody (D-8) is available as both the non-conjugated anti-PLC beta 1 antibody form, as well as multiple conjugated forms of anti-PLC beta 1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC b1, PLC b2, PLC b3, PLC b4, PLC g1, PLC g2, PLC d1, PLC d2 and PLCe. PLC g1 is widely distributed in bronchiolar epithelium, type I and II pneumocytes and fibroblasts of the interstitial tissue. Actin-regulatory protein villin is tyrosine phosphorylated and associates with PLC g1 in the brush border of intestinal epithelial cells. Villin regulates PLC g1 activity by modifying its own ability to bind phosphatidylinositol 4,5-biphosphate. PLC g1 binds a1b1 integrin and modulates a1b1 integrin-specific adhesion. PLC g1 and Ca2+ play a direct role in VEGF-regulated endothelial growth, however this signaling pathway is not linked to FGF-mediated effects in primary endothelial cells. PLC g1 is rapidly activated in response to growth factor stimulation and plays an important role in regulating cell proliferation and differentiation. It may also have a protective function during cellular response to oxidative stress.