mouse monoclonal IgG1; Akt1 Antibody (B-1) is an IgG1 κ mouse monoclonal Akt1 antibody (also designated PKB antibody, PRKBA antibody or RAC-ALPHA antibody) that detects the Akt1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA. Akt1 Antibody (B-1) is available as both the non-conjugated anti-Akt1 antibody form, as well as multiple conjugated forms of anti-Akt1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-βb) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. Activation is dependent on PDGFR-β Tyr residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by Insulin or Insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity. The phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in Insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.