mouse monoclonal IgG1; VEGF Antibody (C-1) is an IgG1 κ mouse monoclonal VEGF antibody (also designated VEGFA antibody) that detects the VEGF protein of mouse, rat and human origin by WB, IP, IF and IHC(P). VEGF Antibody (C-1) is available as both the non-conjugated anti-VEGF antibody form, as well as multiple conjugated forms of anti-VEGF antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factors (FGFs), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. In staining applications, the VEGF Antibody (C-1) shows cytoplasmic staining. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.