mouse monoclonal IgG1; ACOT8 Antibody (C-3) is an IgG1 κ mouse monoclonal ACOT8 antibody (also designated ACOT8 antibody) that detects the ACOT8 protein of mouse, rat and human origin by WB, IP, IF and ELISA. ACOT8 Antibody (C-3) is available as both the non-conjugated anti-ACOT8 antibody form, as well as multiple conjugated forms of anti-ACOT8 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. ACOT8 (Acyl-CoA thioesterase 8), also designated TEII p35 or hTE, is a novel human thioesterase known to interact with the HIV protein Nef using yeast two-hybrid screening. Nef is an auxiliary gene of the human immunodeficiency virus (HIV) which facilitates virus replication and enhances infectivity. The roles of Nef in HIV-infected cells are likely mediated by specific interactions with cellular proteins. The interaction between Nef and ACOT8 is correlated with CD4 downregulation, suggesting that ACOT8 may be involved in Nef-mediated CD4 downregulation in HIV-infected cells. ACOT8 is 42% identical to thioesterase II from Escherichia coli, and has no significant homology with the two types of animal thioesterases that have previously been cloned (type I and type II thioesterases).