mouse monoclonal IgG1; GADD 45α Antibody (4T-27) is an IgG1 κ mouse monoclonal GADD 45α antibody (also designated GADD 45α antibody or GADD 45 alpha antibody) that detects GADD 45α of mouse, rat and human origin by WB, IP and ELISA. GADD 45α Antibody (4T-27) is available as the non-conjugated anti-GADD 45α antibody form, as well as multiple conjugated forms of anti-GADD 45α antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. It is well established that cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, presumably to allow time for DNA repair prior to entry into S and M phase, respectively. The p53 tumor suppressor is required for one such G1 checkpoint and functions to upregulate expression of GADD 45 and p21. p21 functions to inhibit the kinase activity of multiple Cdk complexes, which may account for its suppression of cell growth. GADD 45 binds both Cdks and PCNA, a protein involved in DNA replication and repair. GADD 45 has been shown to stimulate DNA excision repair in vitro and to inhibit entry of cells into S phase. Thus, it has been suggested that GADD 45 may serve as a link between p53-dependent cell cycle checkpoint and DNA repair.