AUG 02, 2022 6:30 AM PDT

A New, Innovative Process for Clarifying and Sterile Filtering Cells for Protein Purification Workflows

SPONSORED BY: Pall Life Sciences

Introduction

Centrifugation and filtration have been widely accepted as techniques required for clarifying complex cell cultures to recover extracellular proteins such as monoclonal antibodies (mAbs). However, these steps can be time consuming and costly for labs growing their cultures in 24-well plates. This scientific brief offers an alternative to the use of centrifugation/filtration/flocculation to clarify and sterilize mammalian cell cultures. It describes an assessment of a new 24-well clarification and sterile filtration plate and a 24-well sterilization-only filter plate for the recovery of proteins present in the supernatant.

You don't want to miss this!
To permanently access this article for free, fill out the short form below.
Access Article
ALREADY ACCESSED THIS ARTICLE? SIGN IN
About the Sponsor
Scientists and engineers at heart, we thrive on helping customers protect people, the environment and our natural resources. We are often called "the original clean technology company" because our products provide clear environmental benefits. Pall fluid management solutions enable customers to purify and conserve water, consume less energy, make alternative energy possible and practical, advance medicine, and minimize emissions and waste. By doing so we're helping to make our world safer, greener and better every day.
You May Also Like
JUN 27, 2022
Microbiology
Giant Bacterium Challenges Our View of Microbes
JUN 27, 2022
Giant Bacterium Challenges Our View of Microbes
A single filament of Ca. Thiomargarita magnifica. can be seen in this image by Jean-Marie Volland
JUN 28, 2022
Immunology
How the Thymus Trains the Immune System
JUN 28, 2022
How the Thymus Trains the Immune System
For centuries, the thymus was dismissed as a vestigial organ. But in the 60s, researchers suggested that it was actually ...
JUL 12, 2022
Cell & Molecular Biology
Did Sexual Reproduction Originate With Fusion in Archaea?
JUL 12, 2022
Did Sexual Reproduction Originate With Fusion in Archaea?
Sperm and egg cells, or gametes, are unusual. Egg cells are some of the largest cell types, and sperm are some of the sm ...
JUL 18, 2022
Neuroscience
Researchers See the Formation of Motor Memories in Mice
JUL 18, 2022
Researchers See the Formation of Motor Memories in Mice
People usually only have to learn how to ride a bicycle once; and once a person learns how, they can get on and start ri ...
JUL 25, 2022
Genetics & Genomics
Genetic Recombination Appears to be Common in the Human Genome
JUL 25, 2022
Genetic Recombination Appears to be Common in the Human Genome
When the genomes of parents come together to create the genome of a child, their DNA recombines; similar parts are rearr ...
JUL 28, 2022
Cancer
Potential Marker for Prostate Cancer Prognosis Identified
JUL 28, 2022
Potential Marker for Prostate Cancer Prognosis Identified
Cytokines, proteins that affect the immune system, can elicit their effects in either a beneficial or an inhibitory capa ...
Loading Comments...