Optimizing mRNA Transfection of Human T-cells

Optimizing gene-delivery strategies for T-cells is crucial to enhance their therapeutic effectiveness.

The study highlighted in this application note focused on improving the transfection of human T-cells with mRNA using the jetMESSENGER^® transfection reagent. The study's specific goals included identifying culture conditions that maximize cell proliferation, transfection efficiency, and cytotoxic activity against cancer cells. To aid T-cell recovery and expansion, the cells were cultured in specialized GMP media, enriched with high-quality cytokines from Sartorius. The Incucyte^® Live-Cell Analysis System and the iQue^® High-Throughput Screening (HTS) cytometer were employed for kinetic visualization and quantitative assessments, which guided the optimization process.

To access the full results, please fill out the form below to download the application note.