Many research laboratories use the ExpiCHO Expression System to transiently produce candidate biomolecules for biologics development. However, after the lead candidate is found, a stable cell clone expressing the protein of interest is in many instances desired. Thermo Fisher Scientific has recently developed tools to help researchers that are interested in generating ExpiCHO-S-derived stable clones capable of producing high titers and scaling up into a large bio-production process. The high costs and time involved to create and adapt stable cell lines into a suitable production medium can be laborious. ExpiCHO Stable Production Medium provides a seamless transition from transient expression to ExpiCHO stable production, requiring minimal to no cell adaptation. We have generated ExpiCHO-S stable cell lines that produce up to 3.5 more protein than stable clones from traditional CHO-S cells. With our new ExpiCHO Stable Production Medium, Thermo Fisher Scientific provides a clear path toward creating higher-producing stable ExpiCHO-S cell lines while supporting the scalability and product quality attributes important for cell line development.