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AUG 17, 2017 10:00 AM PDT

Applications of Counterflow Centrifugal Elutriation (CCE): Revival of an Old Tool

Speaker
  • Post-doctoral Research Associate, Cell and Molecular Biology, St Jude Children's Research Hospital
    Biography
      After completing a Master's degree in Virology in India, Anisha joined Prof. Timothy Chambers at University of Arkansas for Medical Sciences (UAMS), Little Rock, AR, USA, in 2011 for her graduate studies, earning a PhD in biochemistry and molecular biology with her work involving study of mechanisms of cell death induced by microtubule inhibitors in primary acute lymphoblastic leukemia (ALL) cells. It was in collaboration with Dr. Walter Hittelman from MD Anderson Cancer Center, Houston, TX, that she learned about the technique of centrifugal elutriation using Beckman's JE-6 elutriator rotor. Next, she moved to Memphis, TN, in July 2016, to join Associate Member, Joseph T. Opferman's laboratory at St. Jude Children's Research Hospital for a postdoctoral position, where she works on development of anti-cancer strategies in hematological and solid tumors. Her current research mainly focusses on targeting anti-apoptotic BCL-2 family proteins as a strategy to improve survival in ALL and neuroblastoma. Her publications can be accessed on PubMed at the following link: https://www.ncbi.nlm.nih.gov/pubmed/?term=kothari+anisha. She has been awarded the Alan D. Elbein Award and the Bhuvan Endowment Award for Research Excellence from the Department of Biochemistry & Molecular Biology at UAMS for extraordinary research performance during her graduate career. She has also presented her work at many regional and national meetings.

    Abstract

    DATE: August 17, 2017
    TIME: 10:00am PT, 1:00pm ET

    Counterflow centrifugal elutriation (CCE) is a well-known technique used commonly several decades ago in scientific research. In this technique, cells are subjected to two opposing forces, centrifugal force and fluid velocity, which results in the separation of cells on the basis of size and density. With the advent of more sophisticated and automated technologies, the technique of CCE has taken a back-seat. However, CCE can still provide unique knowledge and scientific breakthroughs in research in several areas. There is a need to revive this technology in order to benefit the most from its unique capabilities. This webinar is aimed at describing the technique of CCE and how a wide range of scientific areas can apply this tool to advance their investigations. This technique is especially helpful in studying aspects of primary acute lymphoblastic leukemia (ALL) cells. Under optimal conditions an essentially pure population of cells in G1 phase and a highly enriched population of cells in S and G2/M phases of cell cycle can be obtained in excellent yield. These cell populations are ideally suited for studying cell cycle-dependent mechanisms of action of anticancer drugs and for other applications. The limitations of the technique will also be discussed. The detailed methodology presented should facilitate application and exploration of the technique to other types of cells.
     


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