Assessing CRISPR On-Target Editing and Structural Changes with UDiTaS™ using Tagify™ Reagents

Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeutic potential of genomic editing technologies. We developed a Uni-Directional Targeted Sequencing methodology, UDiTaS^TM, that is quantitative, removes biases associated with variable-length PCR amplification, and can measure structural changes in addition to small insertion and deletion events (indels), all in a single reaction. Editas processes thousands of UDiTaS reactions yearly, however producing a large-scale supply of a Tn5 transposase complexes (enzyme complexed with next generation sequencing adapters) to create UDiTaS sequencing libraries has been challenging. seqWell has provided the solution with their Tn5 Tagify^TM toolkit to generate custom Tn5-adpater complexes to meet our demands. Custom barcoded adapters are provided to seqWell, and optimized, ready-to-use Tn5 transposase/adapter complexes are created and returned. Custom Tagify batches are reproducible allowing us to continue processing thousands of reactions yearly with consistent results.

 

Learning Objectives