Revolutionary sequencing technologies are enabling whole transcriptome profiling of tens to hundreds of thousands of single cells in parallel, in a single experiment. This has led to an explosion of single-cell sequencing experiments across all fields of biology. However, the success and reliability of these methods depends strongly on the quality of the sample. A poor sample quality, for example containing a lot of dead cells, debris, and contamination from cell-free DNA and RNA, impairs sequencing results and also the costs of an experiment. Consequently, the need to dissociate all types of solid tissues into single-cell suspensions, and isolate target cells and nuclei has grown.
Starting experiments with a fully dissociated, debris-free, viable cell suspension is a critical step to gathering quality data; but it can be difficult to achieve without the right tools and expertise. In this webinar, Miltenyi Biotec will discuss the latest solutions, technical expertise and data to attain quality cells and nuclei for single-cell sequencing from virtually any tissue.
1. Learn which steps in a genomics workflow are most likely to impair sample quality and how to improve those procedures
2. Find out how the latest methods for hands-free tissue and cell dissociation can improve your cell and nuclei quality
3. Discover how critical cell and nuclei sorting steps can be designed to have less of an impact on cells by using the right technology to decrease stress and increase cell viability