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APR 20, 2021 1:30 PM PDT

Panel Presentation: Attain successful single-cell sequencing results with the latest cell and nuclei isolation workflows

Sponsored by: Miltenyi Biotec
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Product Manager for Flow Sorting at Miltenyi Biotec
    Biography

      Alina Steinbach studied Biology in Bonn and Heidelberg, Germany. After her postgraduate studies, Alina finished her PhD at the German Cancer Research Center in Heidelberg. In 2017, Alina joined Miltenyi Biotec as a Global Technical Support Specialist, and in September 2020 she started working as a Product Manager for Flow Sorting.

    • Product Manager for Sample Preparation at Miltenyi Biotec
      Biography

        Carina Emery earned a BS in biochemistry from the University of Florida and an MS in life sciences from Northwestern University. At Northwestern she used a variety of mouse models to study the molecular basis of genetic disorders affecting the nervous system. The San Diego resident then spent time in the Core Genomics facility at the University of Illinois at Chicago, where she developed a passion for utilizing the latest genomic technologies to advance research. Carina moved onto becoming a Field Applications Scientist for Bio-Rad Laboratories, supporting their digital PCR and single-cell sequencing systems. Providing technical assistance to a wide variety of researchers afforded her the firsthand knowledge that no matter how advanced your molecular analysis technology, it doesn't necessarily compensate for poor quality starting material. These days, as Product Manager for Sample Preparation at Miltenyi Biotec, the dedicated biotech professional focuses on finding solutions to ensure researchers begin their experiments armed with the best possible materials.


      Abstract

      Revolutionary sequencing technologies are enabling whole transcriptome profiling of tens to hundreds of thousands of single cells in parallel, in a single experiment. This has led to an explosion of single-cell sequencing experiments across all fields of biology. However, the success and reliability of these methods depends strongly on the quality of the sample. A poor sample quality, for example containing a lot of dead cells, debris, and contamination from cell-free DNA and RNA, impairs sequencing results and also the costs of an experiment. Consequently, the need to dissociate all types of solid tissues into single-cell suspensions, and isolate target cells and nuclei has grown.
      Starting experiments with a fully dissociated, debris-free, viable cell suspension is a critical step to gathering quality data; but it can be difficult to achieve without the right tools and expertise. In this webinar, Miltenyi Biotec will discuss the latest solutions, technical expertise and data  to attain quality cells and nuclei for single-cell sequencing from virtually any tissue.
       

      Learning Objectives:

      1. Learn which steps in a genomics workflow are most likely to impair sample quality and how to improve those procedures

      2. Find out how the latest methods for hands-free tissue and cell dissociation can improve your cell and nuclei quality

      3. Discover how critical cell and nuclei sorting steps can be designed to have less of an impact on cells by using the right technology to decrease stress and increase cell viability
       


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