Beyond PCR: Unlocking the potential of isothermal amplification with RCA and RPA.

Isothermal amplification methods streamline nucleic acid amplification without the need for thermal cycling. Join us to explore two innovative techniques, Recombinase Polymerase Amplification (RPA) and Rolling Circle Amplification (RCA), to discover how they enhance downstream analysis and therapeutic applications.

 

RPA operates at a constant low temperature (37-45 °C) with only two specific primers, offering a rapid and sensitive alternative to PCR. In this webinar, we will demonstrate the use of RPA reaction as a low temperature NGS library amplification option.

 

RCA is designed to amplify circular DNA templates from minute amounts of starting material, addressing challenges like low yield and contamination from the host used for plasmid purification. In this webinar, we will demonstrate how RCA can streamline workflows resulting in a significant reduction in experimental time for protein expression and mRNA production. Additionally, we will explore a robust RCA-based method for SNP detection, making it a valuable tool in genetic research and clinical diagnostics.

 

Learning Objectives