DATE: June 17, 2021
TIME: 7:00am (PDT), 10:00am (EDT)
Colorectal cancer is a particularly challenging disease for immunotherapy given tumor heterogeneity. Multiplexed whole slide imaging allows deep contextual analysis of cancer tissue and provides new insights into cancer tissue heterogeneity, including cell-to-cell interactions and the tumor immune microenvironment. In the current study, the Cell DIVE™ multiplexed immunofluorescence solution was used to comprehensively map 36 biomarkers on a single Colon Cancer Tumor Microarray (TMA), with over 96 tissue core samples, enabling multiplexed biomarker labeling of tumors from multiple patients. Stitched images were imported into HALO® image analysis software (www.indicalab.com). For analysis, a subset of 18 biomarkers were used for tissue classification, cell segmentation, cell phenotyping and spatial analysis. Multiplexed tissue immunofluorescence imaging of a multi-patient TMA provides unprecedented and novel insights into cell populations and spatial interactions in the tumor microenvironment across patient samples.
Identify why immunotherapy in colon cancer is challenging.
Explain how multiplexed imaging provides new insights into tumor heterogeneity, enabling tumor stratification, that may help define new markers for future therapy
Examine single cell analysis of multiplexed immunofluorescence Cell DIVE™ images of biomarkers on a single TMA slide arrayed with 96 tissue samples
Explore how precise image processing between staining rounds allows the exploration of biomarker co-expression or phenotypes in single cells across all biomarkers
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