JUN 17, 2021 7:00 AM PDT

Cell DIVE: a robust platform for multiplexed whole slide imaging and single-cell analysis

Speaker

Abstract
DATE:  June 17, 2021
TIME:  7:00am (PDT),  10:00am (EDT)
 
Colorectal cancer is a particularly challenging disease for immunotherapy given tumor heterogeneity. Multiplexed whole slide imaging allows deep contextual analysis of cancer tissue and provides new insights into cancer tissue heterogeneity, including cell-to-cell interactions and the tumor immune microenvironment. In the current study, the Cell DIVE™ multiplexed immunofluorescence solution was used to comprehensively map 36 biomarkers on a single Colon Cancer Tumor Microarray (TMA), with over 96 tissue core samples, enabling multiplexed biomarker labeling of tumors from multiple patients. Stitched images were imported into HALO® image analysis software (www.indicalab.com). For analysis, a subset of 18 biomarkers were used for tissue classification, cell segmentation, cell phenotyping and spatial analysis. Multiplexed tissue immunofluorescence imaging of a multi-patient TMA provides unprecedented and novel insights into cell populations and spatial interactions in the tumor microenvironment across patient samples.
 
Learning Objectives:
 
  • Identify why immunotherapy in colon cancer is challenging.
  • Explain how multiplexed imaging provides new insights into tumor heterogeneity, enabling tumor stratification, that may help define new markers for future therapy
  • Examine single cell analysis of multiplexed immunofluorescence Cell DIVE™ images of biomarkers on a single TMA slide arrayed with 96 tissue samples
  • Explore how precise image processing between staining rounds allows the exploration of biomarker co-expression or phenotypes in single cells across all biomarkers
 
 
Webinars will be available for unlimited on-demand viewing after live event.

Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
SEP 14, 2021 7:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
APR 21, 2021 5:00 PM CEST
APR 21, 2021 5:00 PM CEST
Date: April 21, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Spatial Answers Trilogy - Spatial Answers in Immunology Immunology Researchers share their Spatial Discoveries in SARS-C...
JUL 15, 2021 9:00 AM PDT
JUL 15, 2021 9:00 AM PDT
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
MAR 16, 2021 10:00 AM PDT
C.E. CREDITS
MAR 16, 2021 10:00 AM PDT
Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
Loading Comments...
Show Resources