SEP 29, 2021 12:00 PM PDT

How Can You Characterize Your CRISPR Gene Edits Faster and Easier?

Presented at: CRISPR 2021
Sponsored by: QIAGEN
C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract

Over the past ten years, CRISPR technology has revolutionized genome engineering, as reflected by the recently awarded Nobel Prize. Nevertheless, the process of performing and characterizing editing events can be challenging for both novices and experts. Suboptimal gRNA design and delivery of the CRISPR components into the target cell can severely affect editing efficiency. This, in turn, can make the already laborious process of characterizing the editing event even more time-consuming and inefficient.

To help you detect and validate your gene edits more effectively, we’ve developed an optimized workflow. The new workflow offers everything you need to characterize your editing event faster and easier, from sample preparation to target amplification and analysis.


Learning Objectives:

1. Verify your editing efficiency with confidence using the new sequencing analysis tool.

2. Design PCR and Sanger sequencing primers with ease.

3. Break down technical failures and input quality.


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