JUN 17, 2021 9:00 AM BST

Considerations on the development of quantitative lateral flow assays

Speaker

Abstract

Due to unforeseen circumstances, Klaus Hochleitner is unable to host this webinar as originally planned. Thanks to Dominik de Cassan for covering the session.

 
Quantitative tests require a much more detailed development and validation effort than qualitative tests. The signal at the test line does not only indicate the presence of the analyte above a pre-defined detection limit, it also allows to determine the concentration of the analyte in the sample liquid. Also, sandwich-type lateral flow assay, with two binders specific for different regions of the target in the sample, and a competitive assay containing a binding molecule that binds a very small target molecule competing with the same molecule immobilized on a membrane, offer different levels of signal intensity and concentrations of the target. Typically, these tests are read by an electronic reader system that generates quantitative data.
Join our webinar hosted by Klaus Hochleitner to find out how quantitative lateral flow tests help to reduce uncertainty in your measurement and the opportunities presented by customized materials (especially membranes) and the benefits of investing in very elaborated quality control processes.
Learning objectives
  • Discuss the differences between sandwich and competitive tests and how to choose between them
  • Understand the key considerations in membrane and reagent selection
  • Review the critical processes including conjugation, lamination, dispensing and drying that help to improve the reproducibility of quantitative tests.

 


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