CRISPR-Based Technologies for Pathogen Detection

The Myhrvold lab develops CRISPR-based technologies for studying RNA. Our primary interest is RNAs involved in host-pathogen interactions. We use a variety of RNA viruses and other pathogens as model systems. In collaboration with others, we also apply our technologies in a diverse array of biological contexts. Our goal is to quickly, cheaply, and easily quantify thousands of nucleic acid sequences in hundreds of samples simultaneously. To achieve this goal, we are building on our massively multiplexed nucleic acid detection technology CARMEN (e.g., mCARMEN, qCARMEN, bbCARMEN).We develop CRISPR-based diagnostic assays that are easier to deploy widely, allowing people to perform testing in a distributed fashion. We are inspired by the utility of rapid antigen tests during the SARS-CoV-2 pandemic.

Learning Objectives:

• Describe the CRISPR-based technologies developed for detecting RNA involved in host-pathogen interactions.
• Differentiate between CARMEN, mCARMEN, qCARMEN, and bbCARMEN in terms of their applications for multiplexed nucleic acid detection.
• Discuss the potential of CRISPR-based diagnostics for widespread, distributed pathogen testing.