MAR 20, 2024 8:00 AM PDT

Deciphering Tissue Complexity: Comprehensive Multi-omic Profiling with the SH800 Cell Sorter and ResolveOME™ Amplification

Sponsored by: Sony Biotechnology

Event Date & Time
Date: March 20, 2024
Time: 8:00 AM (PST), 11:00 AM (EST), 4:00 PM (CET)
Deciphering somatic mosaicism in healthy tissues and clonal diversity in tumors necessitates single-cell analysis. High-quality genomic and transcriptomic data at the single-cell level depends on the efficient isolation of individual cells, coupled with robust amplification techniques upstream of sequencing.
In this webinar, we present a methodology using the SH800 Cell Sorter and ResolveOME™ multi-omic single-cell/single-nucleus amplification for comprehensive genomic analysis. Studies using this combinatorial approach to analyze the transcriptional states and mutations in invasive breast cancer cells and to study copy number variation (CNV) profiles from primary neurons, murine brain, and cultured cell lines will be presented.
Overall, the approach of integrating cell and nuclei sorting with multi-omic analysis enables the critical pairing of genotype with phenotype and provides critical insights into tumor evolution and somatic mosaicism in normal tissues.
Learning Objectives
  • Explore how microfluidics-based cell sorting with the SH800 can be used to purify epithelial cells from immune enriched breast cancer samples and sort nuclei from primary cells and cell lines
  • Learn how the ResolveOME™ technology can be used for robust genome and transcriptome amplification that can be used for next generation sequencing and bioinformatic analysis with BaseJumper® computational tools
  • Uncover crucial understandings regarding tumor evolution and somatic mosaicism in normal tissues, thereby enhancing comprehension of cancer heterogeneity and progression
Webinars will be available for unlimited on-demand viewing after live event.

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