DATE: Thursday, June 20, 2019
TIME: 8:00am PDT
Retinal ganglion cells (RGCs) are projection neurons that process and transmit visual information from the retina to the brain. In a group of diseases called optic neuropathies, these cells degenerate, leading to permanent vision loss. We have been interested in using primary mouse RGCs and human induced pluripotent stem cell (iPSC)-derived RGCs to perform functional genomic screens using chemical, siRNA and, most recently, gRNA libraries. Using the Labcyte® Echo® Liquid Handler we have developed a high-throughput method to screen in cultured RGCs. Moreover, we have developed protocols to synthesize chimeric gRNAs and leverage CRISPR technology to perform site-directed mutagenesis in both primary mouse and human iPSC-derived RGCs.
Key Learning Objectives:
Understand how high-throughput screening is being used to identify neuroprotective targets
Learn about the workflow to conduct HTS screens in primary and stem cell-derived neurons
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