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JAN 24, 2018 9:00 AM PST

ELISA: First Principles

Sponsored by: Abcam
Speaker
  • Scientific Support Manager, Abcam
    Biography
      For the past four years Ralph Paul has been Scientific Support Manager in Abcam's Eugene, Oregon office. Ralph has a PhD in viral receptor characterization and internalization mechanisms, and a fellowship studying viral molecular biology. These experiences were followed by a long tenure in biotech working in gene therapy focused on multiple inflammatory diseases and cancers. Through the course of these studies he has developed and run many assay types directed towards assessing both the expression levels and biological activity of numerous therapeutic proteins and their targets.

    Abstract

    Enzyme-linked immunosorbent assays (ELISAs) allow users to rapidly and sensitively determine the presence and concentration of a target molecule in a biological sample. In this webinar, we discuss the different ELISA formats and ELISA reagents, as well as core ELISA principles and troubleshooting techniques.

    Learning Objectives:

    • General ELISA principles
    • The differences between types of ELISAs, including direct ELISA, competitive ELISA, sandwich ELISA, and SimpleStep ELISA®
    • What could go wrong and how to troubleshoot.

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