Establishing a robust 37-marker mass cytometry assay for deep single-cell immune profiling of whole blood

Sponsored by: Miltenyi Biotec

Speakers

Adeeb Rahman, PhD

Director of Technology Development, Human Immune Monitoring Center - Icahn School of Medicine at Mount Sinai New York
BIOGRAPHY
Mona Al-Maarri, PhD

Product Manager Flow Cytometry - Miltenyi Biotec
BIOGRAPHY

Event Date & Time

DATE: July 24, 2019

TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST

Abstract

Mass cytometry (CyTOF®) is a powerful approach to characterize the immune composition of complex biological specimens and gain insights into immune correlates of disease. Successful large-scale CyTOF immune monitoring studies require several important considerations, ranging from panel design to data analysis. In this webinar, we describe our development and optimization of a 37-marker lyophilized antibody panel to profile whole blood samples in a multi-site pediatric allergy immune monitoring study.

Learning Objectives:

Ensuring optimal data quality, including antibody target and clone selection
Understaning Panel Development
Understanding the optimization of a streamlined sample processing and data analysis pipeline

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