Evaluating Nonderivatization & Derivatization Tandem MS Methods for Multianalyte Analysis in Dried Blood Spots

Date:  Wednesday, February 25, 2015
Time(s):  08:00 PST, 11:00 EST, 16:00 UK, 17:00 CET

Flow injection tandem mass spectrometry (FIA-MS/MS) has been frequently used to analyze amino acids (AA), acylcarnitines (AC), and succinylacetone (SUAC) in dried blood spots (DBS) for inborn errors of metabolism (IEM) research. While butyl esterification method (derivatization) is routine, it is possible to detect AAs, ACs, and SUAC as their native free acids (nonderivatization). In this webinar, we will discuss the research regarding evaluation and comparison of nonderivatization and derivatization sample preparation methods on a triple quadrupole mass spectrometer. The use of meta-calculation software for post-analytical data processing in research will also be discussed.

Who Should Attend: due at project initiation

Researchers who are interested in:

• Inborn errors of metabolism (IEM) research


• Quantitation of amino acids and acylcarnitines


• Selected reaction monitoring (SRM) method development and verification using triple quadrupole mass spectrometer


• Streamlining post-analytical data processing


• Nonderivatization vs. derivatization sample preparation method comparison for IEM research

Key Learning Objectives: due at project initiation

What you will learn:

• Benefits of tandem mass spectrometry in inborn errors metabolism (IEM) research


• Workflow of using tandem mass spectrometry for analysis of AAs, ACs, and SUAC in dried blood spots (DBS)


• Challenges of handling huge quantities of data generated from tandem MS analysis


• Discuss a meta-calculation software for post-analytical data processing


• Discuss research results of evaluation and comparison of nonderivatization and derivatization sample preparation methods on a triple quadrupole mass spectrometer

For research use only. Not for use in diagnostic procedures.