Fluorophore fundamentals for flow cytometry

Flow cytometry is a powerful technique for the analysis of multiple parameters of individual cells within heterogeneous populations. Flow cytometry is used in a broad range of applications including immunophenotyping, fluorescent protein detection, rare event analysis, cell health characterization, and more. In this presentation we will review the basics of fluorescence and how to use a fluorescence excitation/emission spectrum, compare conventional vs. full spectrum flow cytometry, and review numerous types of fluorophores listing advantages and challenges of each. This webinar is useful for new and practiced cytometry users interested in learning the general concepts of fluorescence, and details of the commonly used fluorophores behind flow cytometry.

 

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