OCT 21, 2021 8:00 AM PDT

Generating protein reagents to evaluate the antibody and CD8+ T cell responses to SARS-CoV-2

Speaker

Abstract

DATE: October 21, 2021

TIME:  8:00am PDT

 

Coronavirus disease 2019 (COVID-19) is a global health crisis caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and there is a critical need to produce large quantities of high-quality SARS-CoV-2 antigens for characterization and evaluation of antibody responses to SARS-CoV-2. To address this, we optimized expression and purification of two previously reported SARS-CoV-2 Spike (S) protein constructs and show that ExpiCHO-S™ cells produce enhanced yields of both S proteins compared to other expression systems. Our expression and purification strategy yielded high quality material to allow biochemical, biophysical, and structural (cryo-EM) characterization of the SARS-CoV-2 S protein. We also generated protein microarrays using S protein along with other SARS-CoV-2 antigens (i.e., nucleocapsid) to detect SARS-CoV-2 antibodies in COVID-19 convalescent individuals. Our current efforts are focused on evaluating and activating CD8+ T cells that are specific for SARS-CoV-2. To this end we have developed protein biologics that behave similarly to antigen presenting cells and allow us to activate T cells in an antigen specific (i.e., peptide from an antigen is linked to an MHC molecule) manner. These peptide:MHC molecules are then linked to co-stimulatory molecules to generate proteins we refer to as synTac (artificial immunological synapse for T cell activation). We will develop SARS-CoV-2 synTac protein biologics to activate SARS-CoV-2 CD8+ T cells to determine if this will provide long-term immunity against SARS-CoV-2.

 

Learning objectives:

  • Discuss and lean about glycosylated proteins and discuss different expression platforms for glycoproteins. This can be applied to the SARS-CoV-2 S protein and how glycosylated (or hyperglycosylated) S protein can be utilized for downstream clinical and basic science applications.
  • Describe how SARS-CoV-2 antigens have been used to study antibody responses to COVID-19.
  • Demonstrate how synTac protein biologics activate T cells in an antigen specific manner and compare synTac proteins with current immunotherapies.

 

Webinars will be available for unlimited on-demand viewing after live event.

 


Show Resources
You May Also Like
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 08, 2021 9:00 AM PDT
JUN 08, 2021 9:00 AM PDT
Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
DEC 01, 2021 7:00 AM PST
C.E. CREDITS
DEC 01, 2021 7:00 AM PST
Date: December 01, 2021 Time: 7:00am (PST), 10:00am (EST) In the era of immuno-oncology, there is a growing need for the identification of new biomarkers predictive for sensitivity to anti-P...
AUG 24, 2021 11:00 AM PDT
C.E. CREDITS
AUG 24, 2021 11:00 AM PDT
Date: August 24, 2021 Time: 11:00am (PDT), 1:00pm (EDT) Electron-based dissociation mechanisms have shown great promise for advanced characterization of biomolecules. However, routine adopti...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
Loading Comments...
Show Resources