JUL 29, 2020 9:00 AM EDT

Impedance-based assay to evaluate potency of immunotherapy products

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Analytical Development Scientist, Cell and Gene Therapy Catapult
    Biography
      Juan Miguel Sánchez Nieto studied Biotechnology at Francisco de Vitoria University (Madrid, Spain). He moved to London in 2010 where he did an MSc in Molecular Medicine at UCL. Juan Miguel continued his scientific career at Imperial College London, where he completed a PhD in Developmental and Stem Cell Biology. His research was focused on understanding the differential sensitivity to cell death observed at the onset of embryonic stem cell differentiation. He joined Cell and Gene Therapy Catapult´s Industrialisation team in 2017 as an Analytical Development Scientist. Juan Miguel has been involved in the experimental design and execution of multiple projects related to the development of novel analytics for viral vector, stem cell and somatic cell therapies. Juan Miguel has been leading Cell and Gene Therapy Catapult's efforts in the development and qualification of in vitro potency assays for TCR and CAR-T cell-based immunotherapies.

    Abstract

    Advances in genetic engineering have improved specificity, safety and potency of T-cell receptor (TCR) and Chimeric Antigen Receptor (CAR-T) based therapies for solid and non-solid tumours. These developments have allowed the manufacturing and commercialisation of these immunotherapy ATPMs (advanced therapy medicinal products). However, there is a current need to develop more robust product release assays to shorten product release time, including methods to ensure the identity, safety or potency of the product. Current methodologies to evaluate product potency require cell labelling, are labour intensive and only provide information about product’s specific cytotoxic effect at one selected time-point. The xCELLigence platform overcomes these limitations by measuring electrical impedance over time. In this presentation we will discuss how to develop and qualify the use of a high-throughput impedance-based system to measure T-cell cytotoxicity for both TCR or CAR-T approaches using either suspension or adherent target cell lines.


    Show Resources
    You May Also Like
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    FEB 24, 2021 10:00 AM PST
    C.E. CREDITS
    FEB 24, 2021 10:00 AM PST
    DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
    SEP 03, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 03, 2020 9:00 AM PDT
    DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
    DEC 03, 2020 4:30 PM PST
    C.E. CREDITS
    DEC 03, 2020 4:30 PM PST
    This drug development program is designed to create a family of broad-spectrum, pan-coronaviral drugs that respectively inhibit multiple key enzymes required for viral replication. By target...
    JAN 21, 2021 8:00 AM PST
    JAN 21, 2021 8:00 AM PST
    Date: January 21, 2021 Time: 8:00am (PST), 11:00am (EST) Today, critical reagent characterization is a key component in the overall workflow to establish robust ligand binding assays (e.g.,...
    NOV 05, 2020 7:00 AM PST
    C.E. CREDITS
    NOV 05, 2020 7:00 AM PST
    DATE: Date needed, 2020 TIME: Time needed Exosomes are a population of naturally occurring mobile, membrane-limited, 30 – 100 nm in diameter, extracellular vesicles containing a large...
    Loading Comments...
    Show Resources