Influenza vaccine viral protein profiling for viral potency

In this talk today we will discuss a new approach to profile influenza proteins. Influenza vaccines typically consist of either attenuated, in-activated virus or virosomes which are virus- like particles, which contain antigenic proteins. The viral potency is normally measured by quantitation of a protein hemagglutinin (HA) which is a glycoprotein embedded in the viral bilayer and on the virus outer surface contained in the virosomes.

The solution demonstrated in this webinar uses commercially available kits and prebuilt cartridges together with capillary electrophoresis (CE) to profile the influenza viral proteins. It is based on the separation of viral proteins by capillary gel electrophoresis with sodium dodecyl sulphate (CE-SDS) which is an application which has traditionally been used in quality control of monoclonal antibodies produced by biopharmaceutical companies for over 15 years.

 

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