Genome engineers can leverage an ever-expanding array of biological and computational tools to make targeted genetic changes. Maintaining and optimizing components ranging from reagent design and selection to data analytics has become critical for successful experimental design and intended editing outcomes. In this workshop, we present an end-to-end CRISPR-Cas genome editing paradigm, focusing on applications and improvements at various procedural steps. We will cover key workflows, including target identification through knockout libraries, optimization for targeted genetic changes through homology directed repair (HDR), off-target editing quantification, and more. In addition, we will showcase innovations from IDT’s research and development team.
1. Discover how to optimize oligo and experimental design early in project ideation.
2. Identify ideal methods and reagents to improve the efficiency of generating intended editing outcomes.
3. Discuss the latest IDT R&D developments aimed at improving genome engineering workflows.