Isolation, detection, and analysis: Comprehensive strategies for exosome and extracellular vesicle research with automation enablement

 

Liquid biopsy, a noninvasive method used for analysis of circulating biomarkers like tumor cells, DNA, and exosomes from bodily fluids, shows promise in advancing cancer research for future diagnostic and treatment applications. Exosomes, 30-150 nm extracellular vesicles containing bioactive molecules, play crucial roles in intercellular communication and can indicate the physiological and pathological status of parent cells. Here, we explore workflow capabilities from exosome enrichment to separation detection and analysis with various techniques allowing for flexibility of application, sample throughput, and target size, and analysis. We discuss enrichment and separation techniques for a range of workflows and productivity requirements including magnetic bead isolation of RNA markers within extracellular fluid capable of automation deployment and sorting, sorting of such cells. We then explore cellular sorting advancements with techniques optimized with fluorescent color gating for EVs by flow cytometry or transmission electron microscopy (TEM) imaging. Automation of small particle sorting and characterization can further complement a sensitive workflow and need in cancer therapeutic research and cellular research applications. Join us as we discuss critical considerations for these techniques to enable vital research capabilities.

 

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