DATE: May 24, 2018
TIME: 9:00AM PDT, 5:00PM CET

Isolation of tumor cells and tumor-infiltrating leukocytes for optimized downstream analysis

Recent advances in immuno-oncology research have proven the critical role of immune cells in cancer therapy. Deep understanding of the interaction and phenotype of immune and tumor cells within the tumor microenvironment is key to developing future immune based cancer therapy strategies. However, the most significant challenge to working with solid tumors is the need to dissociate the samples prior to their use in downstream applications, such as immunomagnetic enrichment, flow cytometry, and single-cell sequencing. Furthermore, tumor cells can cause background signals and introduce bias to the analysis of immune cells.

Learning Objectives:

•    Optimized and automated dissociation of tumor tissue resulting in high yield, viability, and improved cell surface marker detection

•    Analysis of tumor cells by flow cytometry

•    Isolation of tumor subpopulations for enhancing NGS based analyses

•    Isolation and analysis of tumor-infiltrating immune cell subpopulations (TILs)