In this talk we will cover basic principles of KASP® genotyping chemistry and designing assays that are specific to desired targets. We will go over commonly encountered challenges such as targeting highly conserved regions of genomes to polyploidy and distinguishing targets on chromosomes that are part of a collective of similar organisms, e.g. wheat. We will start with the basics of BLAST for highlighting regions to avoid and move on to the annotation of these regions for submission to LGC for design. From simple diploids such as human and tomato to more complex genome structures such as potato and wheat, we will show real examples before and after analysis.

Learning Objectives:

• Become familiar with how KASP works and how different design strategies can be used to answer a multitude of research questions.

• Apply publicly available sequence analysis tools to solve common problem faced when designing primers and translating this for sequence submission with worked examples.

• Show how these simple design principles can be used to answer more complex genetic questions such as differentiating and selectively target loci in polyploid genomes or targeting large insertions / deletions / rearrangements using worked examples.