MENU
SEP 15, 2015 7:00 AM PDT

WEBINAR: Less False Negatives: Quantifying Cell Viability by Simultaneous Triple Staining

Speakers
  • Research Scientist, Kroemer Lab, Paris, France
    Biography
      Oliver Kepp received his Ph.D. in 2006 from the Humboldt University of Berlin and the Max Planck Institute for Infection Biology in Berlin, Germany. He is currently a research scientist in the laboratory of Guido Kroemer, where he investigates several aspects of immunogenic cell death, focusing on systems biology approaches.
    • Research Engineer, Institut Gustave Roussy, Villejuif, France, Kroemer Lab, Paris, France
      Biography
        Allan Sauvat received his engineer's degree in 2011 from the Institut Supérieur de BioSciences in Paris, France. He is now a research engineer on the BioCell platform in Villejuif, France, where is focusing on HTS assay and software development for systems biology.

      Abstract
      Cellular viability is usually determined by measuring the capacity of cells to exclude vital dyes such as 4',6-diamidino-2-phenylindole (DAPI), or by assessing nuclear morphology with chromatinophilic plasma membrane-permeant dyes, such as Hoechst 33342. However, a fraction of cells that exclude DAPI or exhibit normal nuclear morphology have already lost mitochondrial functions and/or manifest massive activation of apoptotic caspases, and hence are irremediably committed to death. Here, we developed a protocol for the simultaneous detection of plasma membrane integrity (based on DAPI) or nuclear morphology (based on Hoechst 33342), mitochondrial functions (based on the mitochondrial transmembrane potential probe DiOC6(3)) and caspase activation (based on YO-PRO®-3, which can enter cells exclusively upon the caspase-mediated activation of pannexin 1 channels). This method, which allows for the precise quantification of dead, dying and healthy cells, can be implemented on epifluorescence microscopy or flow cytometry platforms and is compatible with a robotized, high-throughput workflow.

      Learning Objectives:
      • Learn about the protocol developed for the simultaneous detection of plasma membrane integrity (based on DAPI) or nuclear morphology (based on Hoechst 33342), mitochondrial functions (based on the mitochondrial transmembrane potential probe DiOC6(3)) and caspase activation (based on YO-PRO®-3, which can enter cells exclusively upon the caspase-mediated activation of pannexin 1 channels)
      • Learn how this method, which allows for the precise quantification of dead, dying and healthy cells, can be implemented on epifluorescence microscopy or flow cytometry platforms and is compatible with a robotized, high-throughput workflow

      Show Resources
      You May Also Like
      AUG 18, 2020 10:00 AM PDT
      C.E. CREDITS
      AUG 18, 2020 10:00 AM PDT
      DATE: August 18, 2020 TIME: 10:00am PT Get deeper understanding of gene expression patterns by using assays that retain spatial organization at single cell resolution! Come learn about the n...
      NOV 10, 2020 7:00 AM PST
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      NOV 10, 2020 7:00 AM PST
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      DATE: November 10, 2020 TIME: 7:00am PDT, 10:00am EDT Automation can provide tremendous benefits such as increased pipetting precision and accuracy, productivity, and throughput. Numerous wo...
      APR 07, 2020 8:00 AM PDT
      C.E. CREDITS
      APR 07, 2020 8:00 AM PDT
      DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
      SEP 02, 2020 7:00 AM PDT
      C.E. CREDITS
      SEP 02, 2020 7:00 AM PDT
      DATE: September 2, 2020 TIME: 03:00pm PDT, 6:00pm EDT Spatial omics is an expanding collection of methods to examine biological molecules in their geographical context. By retaining the prec...
      MAY 08, 2020 10:00 AM PDT
      C.E. CREDITS
      MAY 08, 2020 10:00 AM PDT
      DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
      SEP 10, 2020 9:00 AM PDT
      C.E. CREDITS
      SEP 10, 2020 9:00 AM PDT
      Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
      Loading Comments...
      Show Resources
      Attendees
      • See more