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MAY 19, 2021 8:00 AM PDT

Turn on the lights in cryo-FIB tomography sample preparation

Speaker

Abstract

Date:  May 19, 2021

Time: 8:00am PDT

 

Cellular cryo-electron tomography is a high-resolution technique that enables imaging of the molecular machinery of a cell at close-to-native conditions. To render cells suitable for tomographic imaging, electron-transparent regions, named cryo-lamellae, must first be prepared with a cryo-focused ion beam (cryo-FIB) microscope. These cryo-lamellae are essentially snapshots of functional cellular environments preserved in a fully hydrated state and embedded in non-crystalline vitreous ice. The process of lamella preparation has now been further developed and automated. To enable correlative imaging of vitrified samples, a new integrated light microscope has been added to the cryo-FIB microscope. Now, the correlation of signals between two imaging modalities - light and electron microscopy - within one system becomes possible. The built-in fluorescence module facilitates localization of lamella sites and makes it possible to verify whether already milled lamellae contain fluorescent targets. In this webinar we will share with you the latest developments and advances.

 

Learning Objectives:

  • How cryo-electron tomography is used for imaging the interior of cells at high resolution
  • How thin electron-transparent windows (termed cryo-lamellae) are milled into cryogenically frozen (vitrified) cells
  • How a new fluorescence microscope that is integrated into the Aquilos 2 cryo-FIB facilitates correlative imaging and lamella targeting

 

 

 

Webinars will be available for unlimited on-demand viewing after live event.

 

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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