OCT 20, 2021 10:00 AM PDT

Mastering Complex Samples: Simultaneous Analysis of Aflatoxins and Sterigmatocystin Using a NEW Immunoaffinity Column Sample Prep with LC

C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract

Aflatoxins are carcinogenic mycotoxins that have adverse health effects on both humans and animals consuming contaminated food and feed, respectively. A method has been developed for the highly sensitive and selective determination of regulated aflatoxins in a wide range of commodities. The extraction of aflatoxins from representative commodities of interest (corn, wheat, oats, red chili, black pepper, cocoa and a traditional Chinese medicine) was performed using liquid-liquid extraction and then immunoaffinity column clean-up on the new AflaTest WB SR+ column. Chromatographic separation was demonstrated using both HPLC (Alliance) and UPLC (ACQUITY UPLC H-Class PLUS) platforms, using fluorescence detection, supported with post-column derivatization and large flow cell, respectively. The performance of the method was evaluated through replicate analysis of spiked test portions of seven different matrices. Overall recovery was shown to be excellent, between 93% and 116%, with relative standard deviations lower than 8%. The method was found to be specific as no interference peaks were observed for blank samples. The method has been demonstrated as suitable for monitoring compliance with regulatory limits set for aflatoxins in food commodities globally.

Learning Objectives:

1.    Describe the importance to test for mycotoxins.

2.    Explain the difficulties encountered when analyzing complex matrices.

3.    Break down what solution(s) does Waters-VICAM offer for Aflatoxin testing in complex matrices.


Show Resources
You May Also Like
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
C.E. CREDITS
In-house testing is not required federally; however, states differ in their requirements. Cannabis extraction laboratories need to test extracts for pesticides, heavy metals, residual solven...
DEC 01, 2021 7:00 AM PST
C.E. CREDITS
DEC 01, 2021 7:00 AM PST
Date: December 01, 2021 Time: 7:00am (PST), 10:00am (EST) In the era of immuno-oncology, there is a growing need for the identification of new biomarkers predictive for sensitivity to anti-P...
SEP 16, 2021 8:00 AM PDT
C.E. CREDITS
SEP 16, 2021 8:00 AM PDT
Date: September 16, 2021 Time: 8:00am (PDT), 11:00am (EDT) Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. T...
DEC 07, 2021 10:00 AM PST
C.E. CREDITS
DEC 07, 2021 10:00 AM PST
Date: December 07, 2021 Time: 10:00am (PST) The RNAscope™ technology allows high sensitivity and spatial resolution providing pivotal single-cell information to gain better insights in...
Loading Comments...
Show Resources