JUL 29, 2020 4:00 PM EDT

Measuring Immune Cell Killing Dynamics through Real Time Impedance and Live Cell Imaging

C.E. Credits: Florida CE P.A.C.E. CE
Speaker

Abstract

The cytolytic activity of Immune cells toward cancer cells is finely regulated through several pathways and receptors. Although end point assays have been routinely used to measure cell killing, they have intrinsic limitations which led to new in vitro potency assays that are more sensitive and better predictive of in vivo outcomes. The introduction of kinetic approaches has tremendously expanded the dynamic range and resolution of potency determination, allowing the development of safer drugs. In this webinar, Dr. Cerignoli will provide an overview of the Agilent xCELLigence Real Time Cell Analysis technology and its utility in cell mediated cytolysis.  He will describe the advantages and limitations of impedance and imaging alone approaches and highlight the importance of comprehensive multiparametric analysis.

For Research Use Only. Not for diagnostic procedures.

 

Learning Objectives:

1. Comprehend advantages of combining real time impedance and live cell imaging in your immuno-oncology workflow

2. Identify Important factors to consider when designing cell-mediated cytolysis xCELLigence experiments 3. Understand specific cellular phenomena from kinetic data analyzed with RTCA Software


Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
JUN 24, 2021 10:00 AM PDT
C.E. CREDITS
JUN 24, 2021 10:00 AM PDT
Date: June 24, 2021 Time: 10:00am PDT The Chan Zuckerberg Initiative (CZI) was founded to help solve some of society’s toughest challenges— from eradicating disease and improving...
APR 21, 2021 10:00 AM PDT
C.E. CREDITS
APR 21, 2021 10:00 AM PDT
Date: April 21, 2021 Time: 10:00am (PDT), 1:00pm (EDT) Serological testing for SARS-CoV-2 has been steadily adopted into clinical practice over the course of this pandemic. In this webinar,...
Loading Comments...
Show Resources