JUL 29, 2020 4:00 PM EDT

Measuring Immune Cell Killing Dynamics through Real Time Impedance and Live Cell Imaging

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Senior Field Application Scientist, Agilent Technologies
    Biography
      Dr. Cerignoli is covering the role of Senior Field Application Scientist for the xCELLigence RTCA platform at Agilent Technologies, where he is involved in developing new tools and reagents and in validating the xCELLigence technology across multiple cellular models. He received his Ph.D. in Molecular Pathology from the University of Rome "La Sapienza" in Italy and moved to San Diego to study signal transduction and stem cell differentiation at the Sanford Burnham Medical Research Institute. Before joining Agilent Technologies, Dr. Cerignoli worked with other San Diego-based Biotechnology Companies where he was awarded several NIH small business grants to develop new tools to study compound-mediated cardiotoxicity

    Abstract

    The cytolytic activity of Immune cells toward cancer cells is finely regulated through several pathways and receptors. Although end point assays have been routinely used to measure cell killing, they have intrinsic limitations which led to new in vitro potency assays that are more sensitive and better predictive of in vivo outcomes. The introduction of kinetic approaches has tremendously expanded the dynamic range and resolution of potency determination, allowing the development of safer drugs. In this webinar, Dr. Cerignoli will provide an overview of the Agilent xCELLigence Real Time Cell Analysis technology and its utility in cell mediated cytolysis.  He will describe the advantages and limitations of impedance and imaging alone approaches and highlight the importance of comprehensive multiparametric analysis.

    For Research Use Only. Not for diagnostic procedures.

     

    Learning Objectives:

    1. Comprehend advantages of combining real time impedance and live cell imaging in your immuno-oncology workflow

    2. Identify Important factors to consider when designing cell-mediated cytolysis xCELLigence experiments 3. Understand specific cellular phenomena from kinetic data analyzed with RTCA Software


    Show Resources
    You May Also Like
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    FEB 24, 2021 10:00 AM PST
    C.E. CREDITS
    FEB 24, 2021 10:00 AM PST
    DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
    SEP 03, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 03, 2020 9:00 AM PDT
    DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
    DEC 03, 2020 4:30 PM PST
    C.E. CREDITS
    DEC 03, 2020 4:30 PM PST
    This drug development program is designed to create a family of broad-spectrum, pan-coronaviral drugs that respectively inhibit multiple key enzymes required for viral replication. By target...
    JAN 21, 2021 8:00 AM PST
    JAN 21, 2021 8:00 AM PST
    Date: January 21, 2021 Time: 8:00am (PST), 11:00am (EST) Today, critical reagent characterization is a key component in the overall workflow to establish robust ligand binding assays (e.g.,...
    NOV 05, 2020 7:00 AM PST
    C.E. CREDITS
    NOV 05, 2020 7:00 AM PST
    DATE: Date needed, 2020 TIME: Time needed Exosomes are a population of naturally occurring mobile, membrane-limited, 30 – 100 nm in diameter, extracellular vesicles containing a large...
    Loading Comments...
    Show Resources