Monoclonality Assurance Using CloneSelect Single-Cell Printer and CloneSelect Imager in Human Stem Cell (hSC) Engineering Workflow

In recent years regenerative cell therapy utilizing the pluripotency of hESCs or iPSCs is gaining its ground and holds promise to outpace conventional treatment options for cardiac, kidney, liver, neuronal and other diseases in years to come. However, engineering and maneuvering these very unique cell types remain challenging. While the regulatory guidelines for this novel therapeutic modality are still being worked out, we introduced CloneSelect Single-Cell Printer and CloneSelect Imager in hSC workflow and established a distinct roadmap. This enabled high throughput screening of engineered iPS and ES clones by preserving traceability with assurance of monoclonality while maintaining their normal genetic integrity, pluripotency and cellular functionality. The implementation of these technologies enabled to build a unique collection of hundreds of clonal iPSC and ESC lines aiding to deeper understanding of products and processes associated with regenerative therapies.

 

Learning Objectives