JUL 13, 2021 11:00 AM PDT

Monoclonality Assurance Using CloneSelect Single-Cell Printer and CloneSelect Imager in Human Stem Cell (hSC) Engineering Workflow

Sponsored by: Molecular Devices
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Scientist II in AstraZeneca in Stem Cell Therapy team in the Department of Bioprocess Development
    BIOGRAPHY

Abstract
Date:  July 13, 2021
Time: 11:00am (PDT),  2:00pm (EDT)
 
In recent years regenerative cell therapy utilizing the pluripotency of hESCs or iPSCs is gaining its ground and holds promise to outpace conventional treatment options for cardiac, kidney, liver, neuronal and other diseases in years to come. However, engineering and maneuvering these very unique cell types remain challenging. While the regulatory guidelines for this novel therapeutic modality are still being worked out, we introduced CloneSelect Single-Cell Printer and CloneSelect Imager in hSC workflow and established a distinct roadmap. This enabled high throughput screening of engineered iPS and ES clones by preserving traceability with assurance of monoclonality while maintaining their normal genetic integrity, pluripotency and cellular functionality. The implementation of these technologies enabled to build a unique collection of hundreds of clonal iPSC and ESC lines aiding to deeper understanding of products and processes associated with regenerative therapies.
 
Learning Objectives
  • CloneSelect Single-Cell Printer is an effective tool for single cell isolation of IPSCs and ESCs.
  • Combination of CloneSelect Single-Cell Printer and CloneSelect Imager offers confirmation of monoclonality assessments.
  • Clonal ESCs and iPSCs isolated by CloneSelect Single-Cell Printer maintain genomic stability and pluripotency for many generations and can be effectively programmed to differentiate into three germ layers and organ specific cell types.
 
 
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