OCT 08, 2020 6:00 PM PDT

Multimodal analysis of circulating cell-free RNA (ccfRNA), cell-free DNA (ccfDNA), genomic DNA (gDNA) and CTCs from blood samples in cancer research

Sponsored by: QIAGEN
C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract

While circulating cell-free DNA (ccfDNA) and to some extend CTCs from blood are routinely used as analyte in liquid biopsy cancer research applications, circulating cell-free RNA (ccfRNA) has recently also gained relevance for biomarker studies. The combination of insights from different analytes promises an increase in understanding molecular processes of tumor biology. However, there are still challenges to overcome in the preanalytical workflow which e.g. includes defining suitable blood collection tubes, transport concepts or extraction methods. In this presentation, we show the multimodal use of the PAXgene Blood ccfDNA Tube for plasma extraction as part of a wholistic liquid biopsy workflow.

Learning Objectives:

1. Workflow standardization in Liquid biopsy research

2. ccfDNA stabilization

3. Multmodality in liquid biopsy research 


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