SEP 14, 2021 9:45 AM PDT

Multiplexed proteomics to measure protein dynamics during cell stress

C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract

Cells are continuously exposed to various stresses originating from their own function, environmental changes, and pathogens. To understand the cellular responses to stresses, molecular systems approaches are required to assess and integrate the various global and molecular changes. Quantitative proteomics has proven particularly powerful to directly monitor underlying changes in protein abundance and modifications.

We focus on applying quantitative proteomics to study stress responses, such as proteostasis perturbation and host-pathogen interactions. To be able to determine the highly dynamic and time resolved effects of stresses, we develop proteomics tools capable of resolving these conditions. Therefore, we focus on approaches that combine pulse-SILAC labeling with tandem mass tag-multiplexing. I will present multiplexed enhanced protein dynamics (mePROD) proteomics that allow to globally monitor protein translation and will show examples of its use to understand cellular translatome changes upon inhibition of the integrated stress response and mTORC1 – two crucial cellular stress hubs. Then I will present data from cells infected with SARS-CoV-2. The use systems biology approaches focusing on multi-level proteomics allowed us to first predict and then validate a number of drugs that prevent SARS-CoV-2 replication in cells and have now moved towards the clinic.

Overall, I will give an overview over novel approaches to measure protein dynamics by proteomics including the required bioinformatics tools for their analyses. I will provide examples of the strength of these methods in studying cellular protein dynamics during stress and infection.


Show Resources
You May Also Like
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
DEC 01, 2021 7:00 AM PST
C.E. CREDITS
DEC 01, 2021 7:00 AM PST
Date: December 01, 2021 Time: 7:00am (PST), 10:00am (EST) In the era of immuno-oncology, there is a growing need for the identification of new biomarkers predictive for sensitivity to anti-P...
SEP 16, 2021 8:00 AM PDT
C.E. CREDITS
SEP 16, 2021 8:00 AM PDT
Date: September 16, 2021 Time: 8:00am (PDT), 11:00am (EDT) Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. T...
DEC 07, 2021 10:00 AM PST
C.E. CREDITS
DEC 07, 2021 10:00 AM PST
Date: December 07, 2021 Time: 10:00am (PST) The RNAscope™ technology allows high sensitivity and spatial resolution providing pivotal single-cell information to gain better insights in...
JUN 24, 2021 8:00 AM PDT
C.E. CREDITS
JUN 24, 2021 8:00 AM PDT
Date: June 24, 2021 Time: 8:00am (PDT), 11:00am (EDT) Cardiovascular disease is a leading health problem, affecting almost 30% of individuals in the developed world, and comprises a wide ran...
Loading Comments...
Show Resources