SEP 22, 2020 11:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar

A Novel Method for Quantitative Western Blotting that Eliminates Errors from the Use of Housekeeping Proteins

Speaker
  • Product Manager, Protein and Cell Analysis, Thermo Fisher Scientific
    Biography

      Alok’s PhD and postdoctoral work focused on understanding the role of cytoskeleton and focal adhesion complexes on cell migration and metastasis of cancer cells, resulting in 19 publications in leading peer-reviewed journals. He has been working as a product management professional for over 10 years, launching several next-generation sequencing (NGS)-based genetic research tests that can assess risks of developing cancer. Currently he is a product manager for the Thermo Fisher Scientific electrophoresis portfolio and has launched several products that help research scientists conduct protein research.


    Abstract

    DATE:  September 22, 2020

    TIME:   11:00am PDT

     

    The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins has been in practice for four decades. In recent years, journal editors and reviewers have requested adoption of methods that improve the accuracy and reproducibility of quantitative western blot data. Protein normalization is a critical step in obtaining reliable and reproducible quantitative western blot data. Total protein normalization is considered the gold standard for quantitative western blotting. Protein normalization of western blots has relied upon housekeeping proteins, which exhibit signal saturation and varying levels of cellular expression. These issues can produce spurious results, leading to erroneous conclusions. In this webinar we will talk about a new protein labeling reagent that offers an attractive alternative to achieve total protein normalization. The Invitrogen™ No-Stain Protein Labeling Reagent is a flexible, accurate, rapid, and reliable method to visualize and normalize proteins on a gel or on a membrane (post-transfer). The No-Stain reagent can covalently bind to all proteins on gels or membranes within 10 minutes, does not require any de-staining steps, and can be instantly visualized using any UV, blue light, or fluorescence imagers. The No-Stain reagent does not require any special gels or reagents and is compatible with existing gel staining and western blotting workflows. We present data and analysis demonstrating the ease of use and improved accuracy and reproducibility of quantitative western blot data with No-Stain Protein Labeling Reagent compared to housekeeping proteins.

     

    Learning Objectives:

    • Describe how No-Stain Protein Labeling Reagent is used for quantitative western blotting.
    • Report the advantages of using No-Stain Protein Labeling Reagent over other available methods for quantitative western blotting.

     

     

    Webinars will be available for unlimited on-demand viewing after live event.

     

    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


    Show Resources
    You May Also Like
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    NOV 10, 2020 7:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    NOV 10, 2020 7:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: November 10, 2020 TIME: 7:00am PDT, 10:00am EDT Automation can provide tremendous benefits such as increased pipetting precision and accuracy, productivity, and throughput. Numerous wo...
    MAY 08, 2020 10:00 AM PDT
    C.E. CREDITS
    MAY 08, 2020 10:00 AM PDT
    DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
    SEP 03, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 03, 2020 9:00 AM PDT
    DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
    JUN 17, 2020 1:30 PM PDT
    C.E. CREDITS
    JUN 17, 2020 1:30 PM PDT
    Understanding the complex interplay between a pathogen and the host response is important to developing effective vaccines and therapeutics. The nCounter® Analysis System and GeoMx®...
    Loading Comments...
    Show Resources
    Event Countdown
    • 0 Days
    • 0 Hours
    • 0 Minutes
    • 0 Seconds
    Attendees
    • See more