MAY 28, 2020 8:00 AM PDT

Novel Roles of Actin Cytoskeleton on Mitochondrial Dynamics and Homeostasis

Sponsored by: Andor
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Research associate at Geisel School of Medicine at Dartmouth
    Biography
      Dr Rajarshi Chakrabarti is a research associate at Geisel School of Medicine at Dartmouth, working as a postdoctoral scientist in the lab of Professor Henry Higgs. Dr Chakrabarti completed his BS in Zoology and MS in Biophysics & Molecular Biology from University of Calcutta. Dr. Chakrabarti then finished his PhD in biochemistry in 2015, where he studied spatio-temporal regulation and function of lipid kinases and in the nucleus.

      In 2015, Dr. Chakrabarti joined Professor Higgs lab as a postdoctoral scientist where became interested in mechanisms of mitochondrial dynamics, more specifically in analysing how actin filaments regulate mitochondria dynamics. For the last four years at Dartmouth, Dr. Chakrabarti has made valuable contributions in the field of mitochondria dynamics and has worked to uncover the cellular and molecular mechanisms of actin regulated mitochondrial dynamics. The results of his work were published in 10 papers in high impact journals, were Dr Rajarshi Chakrabarti has been co-author, main author and even corresponding author.
    • Product Specialist - Life Sciences, Andor Technology
      Biography
        Claudia graduated in Applied Chemistry, has a MSc in Human Molecular Biology, a PhD in Cell Biology, and two postdocs in Cell Biology.
        With more than 15 years' experience in research, Claudia has worked in several areas like cell biology, HIV studies, oncobiology, endocrinology and neurosciences. In 2010 Claudia joined Universidade do Algarve as a facility manager and three years after Claudia become the director of Advanced Light Microscopy Facility.
        In May 2019, Claudia joined Andor Technologies as a Product Specialist in the Microscopy Systems Division.
      • Product and Business Manager, Imaris
        Biography
          Meredith is the Imaris Product and Business Manager. She previously worked as an Imaris Sales Engineer from 2010-2017. Meredith received a MS in Biology from the University of Oregon and studied cell signaling and tissue polarity in Mark Peifer's lab at the University of North Carolina at Chapel Hill.

        Abstract
        DATE:  May 28, 2020
        TIME:   8AM PT, 11AM ET, 4PM GMT, 5PM CET
         
        Mitochondria have traditionally been viewed as energy-generating organelles, through oxidation of metabolic substrates and creation of a proton gradient across the inner mitochondrial membrane (IMM), that is utilized to generate ATP. However, it is increasingly clear that mitochondria communicate frequently with the rest of the cell and are therefore important signaling organelles. Mitochondria are extremely dynamic organelles; that fuse and divide continuously. Failures in mitochondrial dynamics cause severe disorders, and therefore the study of such mechanisms has significant importance.
         
        A growing number of studies suggests that actin polymerization participates in mitochondrial communication and dynamics in mammalian cells. We find that there are at least two distinct modes by which actin and mitochondria interact.
        • In one mode (Chakrabarti et al (2019) J. Cell Biol. 217(1): 251-268), elevation of cytosolic calcium activates the endoplasmic reticulum (ER)-bound formin INF2, and the resulting actin polymerization stimulates two distinct events that promote mitochondrial division: increased transfer of calcium from ER to mitochondria, (resulting in IMM constriction); and Drp1 recruitment to the outer mitochondrial membrane (OMM), resulting in OMM constriction.
        • In the other mode (Fung et al (2019) J. Cell Sci.), mitochondrial depolarization causes rapid accumulation of an extensive ‘shell’ of actin filaments around the depolarized mitochondria, dependent on Arp2/3 complex but independent of INF2. This actin shell selectively and subtly unwraps the ER from mitochondria and delays the initial phases of the Pink/Parkin dependent mitophagy pathway.
        To achieve the mechanistic information on actin’s role in mitochondrial homeostasis, we have used ANDOR Dragonfly ultra-fast imaging system. Dragonfly provides the perfect solution for imaging live or fixed mitochondria.
         
        In this webinar, we will present our recent results about on the role of actin cytoskeletal on mitochondrial dynamics. Mitochondria imaging analysis tools will also be discussed.
         
        Learning Objectives:
        • Discover the role of ER in regulation mitochondrial dynamics
        • Uncover the role of actin cytoskeleton in stimulating dynamics of both mitochondrial membranes
        • Identify the difficulties in imaging live mitochondria
        • Understand strategies for image live mitochondria
        • Acquire knowledge on biological imaging analysis.
         
        Webinars will be available for unlimited on-demand viewing after live event.
         
        LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.
         
         

         

         


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