MENU
OCT 13, 2021 1:30 PM PDT

Panel Presentation: Advantage of Absolute Quantification of Digital PCR in Wastewater Monitoring QIAGEN's Workflow Solutions for SARS-CoV-2 Surveillance

Sponsored by: QIAGEN
C.E. Credits: P.A.C.E. CE Florida CE
Speakers

Abstract

One significant challenge with SARS-CoV-2 wastewater monitoring is the lack of optimized and standardized sample processing steps, from sample collection to RNA detection. Viral concentration methods, sampling variability, viral genome stability and the sensitivity of the detection assay are all crucial considerations for designing a testing strategy. In this session, we’ll talk about a wastewater monitoring workflow (digital PCR-based), which overcomes variability and inhibitor problems – without relying on standard curves. To tackle the challenges during sample preparation, we’ll be looking at getting higher yields in RNA extraction from difficult samples, and inhibitor removal to reduce the chance of contamination and downstream inhibition. With dPCR, we’ll explain how absolute quantification offers higher precision and detection sensitivity even in complex wastewater samples with inhibitors and varying viral load.


Show Resources
You May Also Like
MAR 23, 2022 11:00 AM PDT
MAR 23, 2022 11:00 AM PDT
Date: March 23, 2021 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEDT) In this presentation, Dr. Middleton will review the development and deployment of large-scale saliva-based COVID-19 test...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
DEC 09, 2021 11:00 AM PST
C.E. CREDITS
DEC 09, 2021 11:00 AM PST
Date: December 09, 2021 Time: 11:00am (PDT), 2:00pm (EDT) The burden of antimicrobial resistance (AMR) has been acknowledged worldwide by leading health institutes. Besides the need for new...
APR 28, 2022 8:00 AM PDT
APR 28, 2022 8:00 AM PDT
Date: April 28, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Human pluripotent stem cells (PSCs) and their derivatives hold great potentials in...
MAR 16, 2022 8:00 AM PDT
C.E. CREDITS
MAR 16, 2022 8:00 AM PDT
Date: March 16, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CET) Handling of potent and/or hazardous substances is commonplace in sev.....
Loading Comments...
Show Resources