SEP 17, 2019 6:00 AM PDT

Post-translational modification enrichment and quantitation for precision medicine and drug development

Speakers
  • Professor of Translational OncoProteomics, Dept. Medical Oncology, and Head of the OncoProteomics Laboratory and Director of the Proteomics Core, Amsterdam University Medical Center
    BIOGRAPHY
  • Project Leader in Proteomics & Mass Spectrometry at Roche in Basel, Switzerland
    BIOGRAPHY

Abstract
DATE:  September 17, 2019
TIME:    6:00 am PDT, 9:00am EDT, 2:00pm BST, 3:00pm CEST
 
Innovative mass spectrometry-based proteomics is a powerful tool for obtaining insights into disease-related cellular signaling pathways, biomarkers, and potential drug targets. This technology is being used to investigate phosphorylation of extracellular and intracellular proteins with the aim of improving early diagnosis and treatment of cancer and neurodegenerative diseases. In addition to phosphorylation studies, this technology can also be used to detect other PTMs including ubiquitylation. For example, E3 ubiquitin ligases represent a challenging, yet promising, class of proteins in drug discovery. In particular, combining the ligases with targeted protein degradation technology opens the door to developing new tools to study protein ubiquitinylation. To this end, the peptide K-ε-GG remnant affinity enrichment approach (PTMScan®) has been adapted for use with an automated liquid handling platform. This allows increased sensitivity and throughput while decreasing sample-to-sample variability in quantitative experiments. This approach has been successfully applied to the identification of downstream substrate protein candidates of disease-relevant E3 ubiquitin ligases. This webinar considers study design for label-free analysis, enrichment strategies, and data analysis involving both preclinical models and clinical samples with a focus on cancer.
 
During this webinar, participants will:
  • Gain an understanding of the use of pTyr and global affinity enrichment workflows for label-free phosphoproteomics
  • Learn about single-sample integrative data analysis workflow (INKA) for kinase activity scoring
  • Consider an automated workflow for modified peptide affinity enrichment using PTM antibodies, its application for the exploration of E3 ligase substrates, and their potential use as target engagement biomarkers

 

 
Webinars will be available for unlimited on-demand viewing after live event.

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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SEP 17, 2019 6:00 AM PDT

Post-translational modification enrichment and quantitation for precision medicine and drug development



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