AUG 04, 2021 8:00 AM PDT

The Power of Autofluorescence Extraction Using Full Spectrum Profiling™

Sponsored by: Cytek Biosciences
C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract
Date:  August 4, 2021
Time: 8:00am (PDT),  11:00am (EDT)
 
 
The intrinsic emission of light by biological structures in cells, known as autofluorescence (AF), has been a problem in flow cytometry data resolution for years. Cells with a high level of AF can interfere with a flow cytometer's ability to detect specific fluorescent signals; this is especially true when quantifying rare markers or dim fluorochromes.

On a conventional flow cytometer, researchers might be forced to use dyes emitting in spectral regions not affected by AF. But with Full Spectrum Profiling™ (FSP™) as found on the Cytek® Aurora, Northern Lights, and Aurora CS, investigators can fully understand AF, extract the AF signal, and ultimately improve their data resolution.

We will explore different examples of samples with high levels of AF that benefit from Cytek's FSP technology. By fully characterizing and subsequently extracting the AF signature across the entire spectrum, we will show how the resolution of dim markers is improved, sometimes even revealing populations previously masked by cellular AF.

 
Learning Objectives
  • Define and understand sources of autofluorescence
  • Understand where autofluorescence signals are detected and how to capture them
  • Learn how to extract autofluorescence to improve resolution
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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