JUN 17, 2020 7:00 AM PDT

Keep It Real - with Native Protein MS

Speakers

Abstract

We present a novel workflow for native MS, using a robust and sensitive LC/MS method using standard LC flow for native protein analysis. Most of the native MS analyses are using a nano-electrospray approach, which faces significant challenges in handling as well as throughput.

The workflow comprised the Agilent 1290 Infinity II LC, the AdvanceBio SEC column, the 6545XT AdvanceBio LC/Q-TOF, and MassHunter BioConfirm software. Examples from ligand-binding proteins, MAb’s as well as non-covalent complexes will be shown using standard-flow conditions, demonstrating charge-state distribution previously only observed with nano-spray.

We have also utilized the Ion Mobility Q-TOF system to determine the Collision Cross Section (CCS) values of the native protein molecules and the result for different mAb classes and biosimiliars will be shown.

For Research Use Only. Not for use in diagnostic procedures.


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