Kinetic antibody internalization assays using live-cell analysis

Monoclonal antibodies (mAb) and antibody-drug conjugates (ADCs) are widely used biological therapeutics.  A key property of these biologics is the extent and rate of internalization into different cells, which governs their efficacy, safety and pharmaco-dynamic profile. As a result, quantifying and comparing the rate of Ab internalization of drug candidates is critical in the biopharmaceutical selection and optimization process.

In this mini-webinar Nicola Bevan discusses:

• How real-time live-cell analysis of antibody internalization can provide additional insight.
• The use of the IncuCyte FabFluor-pH reagent to provide a turn-key approach to antibody labelling.
• Examples of how the assay may be used for characterisation of early stage test antibodies and candidates in a biologics discovery paradigm.