Revealing the role of Mononuclear phagocytes in human pregnancy via automated high purity isolation

Here, we show how a high purity automated isolation from blood and tissue mononuclear phagocytes allow for an accurate investigation to further understand how these changes occur at the cellular level. Flow cytometry, bioenergetics analysis and transcriptomics were used to monitor the immunometabolic profile of mononuclear phagocytes during pregnancy. We can apply this knowledge to other immune cell populations and also to bridge the many gaps in the basic, translational, and clinical immunology of pregnancy.