Revealing the role of Mononuclear phagocytes in human pregnancy via automated high purity isolation

Sponsored by: Miltenyi Biotec

Speakers

Prof. Cathy Thornton, PhD

Professor of Human Immunology, Swansea University
BIOGRAPHY
Salvador Vento Asturias, PhD

Global Product Manager for Cell Separation Reagents, Miltenyi Biotec B.V. & CO. KG
BIOGRAPHY

Event Date & Time

Date: June 09, 2022

Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST)

Abstract

It is known that the immune response in pregnant women undergoes recognized changes that ensure the mother does not reject the fetus. Dysregulation of this dynamic immune adaptation underpins numerous adverse pregnancy outcomes such as miscarriage, preeclampsia, and preterm birth. These normal changes in the immune response also make pregnant women more susceptible to severe infections of influenza, measles, and hepatitis E and affect clinical symptoms of autoimmune diseases. Despite the knowledge of these immune response changes, little is known about how immune cells undergo functional changes during pregnancy.

Here, we show how a high purity automated isolation from blood and tissue mononuclear phagocytes allow for an accurate investigation to further understand how these changes occur at the cellular level. Flow cytometry, bioenergetics analysis and transcriptomics were used to monitor the immunometabolic profile of mononuclear phagocytes during pregnancy. We can apply this knowledge to other immune cell populations and also to bridge the many gaps in the basic, translational, and clinical immunology of pregnancy.

Learning Objectives

Discuss the characterization of monocyte phenotype and function from subsets to mitochondria
Describe how tissue macrophages respond to their environment
Explain how defined cytokine exposures generate discrete macrophage types

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