FEB 08, 2017 1:30 PM PST

Safety and pharmacokinetic profiles of different buprenorphine SR doses in a rabbit model

Speaker

Abstract

DATE: February 8, 2017
TIME: 1:30pm PT, 4:30pm ET

In rabbits, buprenorphine instant release (IR) is commonly used to alleviate pain, especially in surgical models. However, maintenance of IR therapeutic levels requires redosing which significantly increases unnecessary animal handling, restraint and non-compliance issues post-operatively. The development of sustained-release (SR) formulations of buprenorphine in rabbits to deliver constant levels of the drug with adequate plasma concentration and satisfactory analgesia would greatly improve standard of care. In here, we hypothesized that single subcutaneous administration of at least one of three SR (Buprenorphine SRTM Lab, ZooPharm) concentrations (SRLow - 0.06 mg/kg, SRMedium - 0.15 mg/kg, SRHigh – 0.3 mg/kg - n = 3 animals per group) is associated with up to 72 hours of pain relief and more predictable behavior and pain-associated parameters than IR (0.05 mg/kg BID) in a bilateral rabbit knee defect model. In addition to body weight, fecal output, grimace and lameness (0-4, where 0 = continuous weight bearing, 1 = single limb lameness, 2 = multiple limb lameness, 3 = toe touch – non-weight bearing, and 4 = completely non-weight bearing) scales were assessed and scored before and for 72 hours after surgery. No skin reaction to any of the tested SR concentrations was observed within the study phase. Post-operative assessment also revealed no significant changes in body weight across groups. Compared to IR and SRHigh, daily fecal output assessment was higher in rabbits treated with SRLow and SRMedium. Single limb lameness and signs of pain (grimace scale) were observed only in rabbits treated with IR (24 hours) and SRLow (24-48 hours). In fact, rabbits treated with SRLow also received meloxicam (0.3 mg/kg PO SID) within the first 48 hours. Compared to IR, SRLow and SRHigh single administration of SRMedium had little effect in the fecal output and behavior of rabbits enrolled in the selected surgical model. Rabbits treated with IR, SRHigh and SRMedium had similar PK values during the first post-operative hours. In SRHigh and SRMedium, the plasma concentration of the drug remained within the 1ng/ml mark for at least 48 hours. In conclusion, rabbits treated with one dose of SRMedium at 0.15 mg/kg had similar post-operative outcome to the observed in animals treated twice a day with the gold standard drug (IR) and significantly less adverse effects when compared to the observed in the SRLow and SRHigh groups. Further investigation is needed to validate this pain management protocol in other rabbits orthopedic models.

Learning objectives: 

  • Provide an overview of buprenorphine SR pharmacokinetics at different doses and over time.
  • Provide an overview of adverse effects and clinical findings after buprenorphine SR administration.

Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
JUL 15, 2021 8:00 AM PDT
C.E. CREDITS
JUL 15, 2021 8:00 AM PDT
Date: July 15, 2021 Time: 8:00am (PDT), 11:00am (EDT) High dimensional full spectrum flow cytometry grants unprecedented access to previously unattainable parameters in cellular biology. Flu...
NOV 30, 2021 10:00 AM PST
C.E. CREDITS
NOV 30, 2021 10:00 AM PST
Date: November 30, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The prevalence of thyroid disease worldwide has served as a catalyst for healthcare providers to study various tools and methods to...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
FEB 08, 2017 1:30 PM PST

Safety and pharmacokinetic profiles of different buprenorphine SR doses in a rabbit model



Show Resources
Loading Comments...
Show Resources