Simultaneous 5-plex Immunohistochemistry - MSKCC/PPBC experience

Immunohistochemistry (IHC) has been an indispensable tool for daily pathology practice. Multiplex designs have further facilitated its utility with improved efficacy. Several techniques of multiplex IHC are available; one of which is the Discovery Ultra system of Ventana. It allows combination of AP and HRP based chromogens on sequential runs. In this presentation, we will demonstrate our experience with Discovery Ultra system and our pentaplex design as well as a triplex design with new Beta-product HRP based chromogens.